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巅峰生物蛋白酶K说明书

发表时间:2016-3-3 浏览次数:1822  【关闭

 蛋白酶K说明书

产品简介:

  本产品来源于布氏白僵菌(Beauveria brongniartii)的蛋白酶K基因,经过了基因定点突变和色谱纯化。该酶切割脂族氨基酸和芳香族氨基酸的羧基端肽键,可以用于所有蛋白质的降解。此酶经纯化去除了RNA酶和DNA酶活性。蛋白酶K是现有蛋白酶中活性最高的品种,在pH4到pH12.5范围均有活性,反应温度介于0~75℃之间。该蛋白酶K在尿素和SDS中仍然具有降解天然蛋白质的能力,主要的应用行业包括:医疗及基础研究领域:基因诊断试剂盒;基因组DNA提取试剂盒、RNA提取试剂盒中去除DNA和RNA制备中的核酸酶;制备脉冲电泳的染色体DNA;提取组织中非蛋白成分时,降解含有蛋白质杂质;此外,还应用于:皮革工业(皮革软化及精细化、脱毛和软化、蚕丝脱胶),食品、酿造工业(肉类嫩化、酒类澄清)及洗涤工业(酶洗衣粉、洗衣液等)。

具体参数:

来源种属:布氏白僵菌(Beauveria brongniartii)

固体酶比酶活:≥30 U/mg

分子量:28 KDa

产品性状:固体酶为白色冻干粉

纯  度:≥95%(SDS-PAGE)

贮存和使用:

固体酶可室温运输,长期贮存温度需保持4℃以下。正确的贮存条件下,有效期为三年。

打开包装后,请及时使用,以防止吸潮。固体酶溶解后,建议过滤除菌,分装为适当体积,-20℃贮存。

质量控制:

活性单位定义

37℃、pH7.5条件下,每分钟可水解底物酪蛋白生成1μmol酪氨酸的蛋白酶K的量,定义为一个单位(U)

脱氧核糖核酸酶残留

未检测到脱氧核糖核酸酶活性

核糖核酸酶残留

未检测到核糖核酸酶活性

 

 Proteinase K specification

Product introduction:

  Proteinase K exhibits broad substrate specificity. It degrades many proteins in the native state even in the presence of detergents. Proteinase K is isolated from a fungus, Beauveria brongniartii which is able to grow on keratin. The predominant site of cleavage is the peptide bond adjacent to the carboxyl group of aliphatic and aromatic amino acids with blocked alpha amino groups. Proteinase K is a stable and highly reactive serine protease. It is stable in a broad range of environments: pH (4 to 12.5), buffer salts, detergents (0.1-0.5% SDS) and temperature (0 to75℃).Proteinase K is commonly used in molecular biology to digest protein and remove contamination from preparations of nucleic acid. It is also used in leather industry (leather softening, leather refinement, leather depilation and silk degumming), food industry (meat tenderization and wine clarification) and detergent industry. It is highly suited to this application since the enzyme is active in the presence of chemicals that denature proteins, such as SDS and urea, chelating agents such as EDTA, sulfhydryl reagents, as well as trypsin or chymotrypsin inhibitors. Proteinase K is used for the destruction of proteins in cell lysates (tissue, cell culture cells) and for the release of nucleic acids, since it very effectively inactivates DNases and RNases.

Physical Properties:

Appearance (Color): White to Off White

Appearance (Form): Powder

Product name: Proteinase K from Beauveria brongniartii

Purity: 95% (SDS-PAGE)

Units/mg protein: ≥30 U/mg (Enzymatic Activity Unit Definition: One unit will hydrolyze urea denatured hemoglobin to produce color equivalent to 1.0 micromole of tyrosine per minute at pH 7.5 at 37 ℃)

Storage and use:

Long-term storage temperature: -20℃, or not more than 4℃.

The enzyme powder can be transported at room temperature, for short periods.

Under proper storage conditions, the recommended retest period is three years.

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